During acid-catalyzed glycerol production, wastewater was deacidified and degummed to determine the best treatment conditions. When Ca(OH)2 was used as the deacidification agent, the optimum treatment temperature, pH and reaction time were 60℃, 10 and 15 minutes, respectively. After the reaction, the SO42- concentration was 2.3736 mg/L and its removal ratio was 99.97%. Following deacidification, degumming was necessary. For this process, the best degumming flocculant was FeCl3, the optimum temperature was 60°C and the best pH was 6. The final concentration of SO42- was 2.1836 mg/L and the removal ratio could reach up to 99.973%.