Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

header
Reach Us reach to JOCPR whatsapp-JOCPR +44 1625708989
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Original Articles: 2011 Vol: 3 Issue: 3

A Validated RP-HPLC Method for Simultaneous Estimation of Bronopol in Lactulose Solution USP

Abstract

A validated reverse phase high performance liquid chromatography method has been developed for the simultaneous determination of Bronopol in Lactulose Solution USP. Chromatography was carried out on a BDS Hypersil C18 (4.6 mm × 250 mm, 5 μm) using Water:Acetonitrile: Phosphate Buffer in the ratio of 94.5:5:0.5 (v/v/v) as the mobile phase at a flow rate of 1.0 mL/min and eluents were monitored at 214 nm. The average retention time of Bronopol was found to be 8.160 min. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The calibration curve was linear (R2 ≥0.9998) over the range of 4.0-12.0 μg/mL.