Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

Reach Us +44 1625708989
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Original Articles: 2010 Vol: 2 Issue: 6

A simple and sensitive RP-HPLC method for simultaneous estimation of Artesunate and Amodiaquine in combined tablet dosage form


A simple, accurate, sensitive and validated RP-HPLC method for simultaneous determination of Artesunate and Amodiaquine in combined tablet dosag e form has been developed. Separation was carried out on Jasco HPLC system equipped with Hypersil Gold C 18 (250 × 4.6 mm i.d.) using mobile phase of Acetonitrile and 25 mM potass ium dihydrogen phosphate buffer (70:30, v/v) at a flow rate of 1 ml/min using Artemether as internal standard and detection using UV/VIS detector was carried out at 220 nm. Ambient tempera ture conditions were maintained. Results were linear in the range of 250 – 2000  g/mL for Artesunate and 10 – 30  g/mL for Amodiaquine respectively. The method has been succe ssfully applied for the analysis of drugs in pharmaceutical formulation. Results of analysis wer e validated statistically and by recovery studies.