A sensitive spectrophotometric method for the determination of 2-bromo-2-nitropropane 1, 3-diol (bronopol), at mg L-1 level is described. The method is based on the reduction of the nitro group of bronopol to amino group with Zinc/HCl which subsequently diazotized with sodium nitrite in acidic medium and coupled with phloroglucinol to formed orange azo dye in alkaline medium. The dye shows maximum absorbance at 425 nm and obeyed Beer’s law in the range of 10-50 μg bronopol in 1 mL. The important analytical parameters and optimum reaction conditions have been optimized. The Molar Absorptivity and Sandell’s Sensitivity were found to be 4.02×103 litres mol-1 cm-1 (±100) and 0.049 μg cm-2, respectively. The Standard Deviation and the Relative Standard Deviation were found to be ± 0.0073 and 3.63% respectively.The method has been successfully applied to the determination of bronopol in polluted water, grains and vegetables. The method was found to be free from the interference of most of the commonly used pesticide and other common pollutants.