Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2011 Vol: 3 Issue: 3

A new simultaneous RP-HPLC method for development and validation of Lamotrigine tablets


A simple, rapid and precise method was developed for the quantitative determination of lamotrigine in tablets. The method was based on RP-HPLC. Chromatographic separation was performed on a Xterra C18 ( 4.6 X 100 mm ) column using a mobile phase of methanol and Potassium dihydrogen phosphate (50:50v/v) adjusted the pH . The following system conditions were maintained throughout development and validation i.e., flow rate 0.8ml/min, column was maintained at room temperature and the detected by a UV-wave length at 225 nm. The lamotrigine was well resolved on the stationary phase and the retention time was 3.8 minute. The method was validated and shown to be linear for lamotrigine in 5-25 μg /ml. the correlation coefficient for lamotrigine is 0.999 respectively. The method was validated for Precision, Accuracy, LOD and LOQ were determined.

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