Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2010 Vol: 2 Issue: 4

A new method to prepare macroporous poly(GMA-ST) and its application to immobilize ?²-galactosidase

Abstract

Two kinds of macroporous poly(GMA-ST) were synthesized by bulk copolymerization using different pore-forming agents, one only with liquid porogenic agent including cyclohexanol and lauryl alcohol and the other simultaneously with a mixture of cylohexanol, lauryl alcohol and nano-calcium carbonate. After the polymer was smashed, particles with diameters ranging 0.15mm to 0.30mm were taken as the carrier, Scanning electron microscopy (SEM) micrographs showed that both kinds of carrier. Under the optimum conditions, β-galactosidase was immobilized on the carrier obtained above, the enzyme activity, the activity yield and the basic property of the all the immobilized enzyme were determined separately, and satisfactory results were obtained in enzyme activity, activity yield, pH stability, thermal stability, and operational stability. The conclusion obtained indicated that the ploy(GMA-ST) prepared concurrently with liquid and solid porogen was more suitable to immobilize enzyme than that purely with liquid solution as pore-forming agents.