Phytochemical investigation of alcohol extract of seeds of Nigella sativa were isolated a new ethyl substituted glycoside, O-β-D-ethyl glucopyranosyl-(2→4)-O-β-D-ethyl glucopyranosyl-(2→4)- β-D-ethyl glucopyranoside (1) along with three new aliphatic compounds heptadecyl docosanoic acid (2), hexatetracontan -22-ol (3), 21-methyl pentacosa-6,15-dione (4) and one known compound 16-methyl heptadecanoic acid (5). The structural elucidation of the isolated compounds was based primarily on 1D- and 2D- NMR analysis, including COSY, HMBC and HMQC correlations. The compound 1 shows significant activity against lipoxygenase enzyme.