Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

header
Reach Us +44 1625708989
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Lignocellulosic enzymes characterization and scanning electron microscope analysis on rice bran surface structure changes

Author(s): Mirni Lamid, 1M. Anam Al-Arif, Ni Nyoman Tri Puspaningsih, Anita Kurniati, One Asmarani and Sunaryo Hadi Warsito

Rice bran was one of livestock food ingredients with high crude fibers contents, including cellulose and hemicelluloses. Lignocellulosic enzymes such as xylanase and cellulase functioned in degrading cellulose and hemicelluloses. The objectives of this study were to qualitatively screen Actinobacillus sp. (G6) and Bacillus pumilus (G7) which produced xylanase and cellulase, determining molecular weight of xylanase and cellulase enzymes using SDS-PAGE and Zymogram, and analyzing structure changes on rice bran surface after Scanning Electron Microscope treatments using xylanase and cellulase enzymes. The findings of this study indicated xylanolitic indexes for G6 and G7 were 2 and 1.7 respectively while cellulosic index for G6 and G7 were 1.6 and 2. Enzymes produced by Actinobacillus sp. (G6) had molecular weight 25-35 kDa and above 100 kDa while enzymes produced by Bacillus pumilus (G7) had molecular weight 25 kDa up to above 100 kDa. The result of rice bran SEM analysis indicated profile change of the samples before and after enzymes treatment.

PDF

paper.ioReplica watches

agar io

wowcappadocia.com
cappadocia-hotels.com
caruscappadocia.com
brothersballoon.com
balloon-rides.net

wormax io