Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

High-Throughput Liquid Chromatography �?¢�?�?��?�?? Tandem Mass Spectrometric Method for Simultaneous Quantification of Carvedilol and Its Metabolite 4-Hydroxyphenyl Carvedilol in Human Plasma and Its Application to Bioequivalence Study

Author(s): I. Sarath Chandiran, K. N. Jayaveera and Raghunadha Reddy. S

A simple, sensitive and reliable method is described for simultaneous quantification of Carvedilol and its metabolite 4-Hydroxyphenyl Carvedilol in human plasma by using High-throughput liquid Chromatography�?¢�?�?��?�??tandem mass spectrometric method. The method was carried out by on-line coupling of extraction with Cyclone P (50 mm �?�?�?�?? 0.5 mm 50 �?Ž�?¼m) HTLC(High-throughput liquid Chromatography) column by injecting 10�?Ž�?¼L plasma sample and chromatographic separation was performed with ACE C18 (50X4.6 mm, 5�?Ž�?¼m), followed by quantification with mass detector in multi reaction monitoring using ESI as an interface. The method was linear over a concentration range 0.1 to 250 ng/mL with a limit of Quantification of 0.1 ng/mL for both Carvedilol and 4-Hydroxyphenyl Carvedilol. Intra�?¢�?�?��?�?? and inter-day precision were less than 15%, respectively, and the accuracy was in the range of 91.6-106.2%. Stability assessment was also included. The total run time of analysis was 2.5 min. The validated method was successfully applied to bioavailablility and Bioequivalence study. PDF