Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Development and validation of analytical method for simultaneous estimation of mometasone furoate, hydroquinone and tretinoin in topical formulation by RP-HPLC

Author(s): Kinjal Sheliya, Ketan Shah and Pankaj Kapupara

A simple, sensitive, rapid, precise, andaccurate RP-HPLC method has been developed for simultaneousestimation of Mometasone Furoate (MOM), Hydroquinone (HYQ) and Tretinoin (TRE) from their pharmaceutical dosage form. The Chromatographicseparation was achieved on a reversed-phase Inertsil C18 (4.6 mm I.D. × 250 mm, 5 μm) column using a mobile phase consisting of acetonitrile: Methanol (90:10,v/v) at a flow rate of 0.5 mL/min and UV detection at 266 nm. The method showed linearity with correlation coefficient of MOM, HYQ and TRE was 0.998, 0.9953 and 0.9963 over the range of 5-25μg/mL, 50-250μg/mL and 1-5μg/mL respectively. The mean recoveries were found to be in the range of 99.00 –101.00 % for all the components. The method was validated as per the ICH guidelines for linearity, limit of detection, limit of quantification, specificity, accuracy, precision and robustness. The method can be successfully applied for routine analysis of quantitative determination of MOM, HYQ and TRE in pharmaceutical dosage form.